Measurement

Part:BBa_K1379007:Design

Designed by: Ng Siu Wang Edward, Jason Jang, Joo Min Jung   Group: iGEM14_Hong_Kong_HKUST   (2014-09-23)

σx Generator + PcomFA-E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359
    Illegal BsaI.rc site found at 1550


Design Notes

None

Source

- - PcomFA primers sequence was obtained from Wellcome Trust Sanger Institute, a British genomics and genetics research institute.
- iGEM 2014 Hong_Kong_HKUST Team has cloned PomFA from S. pneumoniae strain NCTC7465 by PCR. Then, restriction-ligation with σX generator BBa_K1379006 and BBa_E0240 in pSB1C3 backbone was performed.

References